The Calcineurin Cellular Activity Assay Kit is a complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well microtiter-plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS, human recombinant calcineurin5 is included as a positive control! The RII phosphopeptide substrate, supplied with this kit, is the most efficient and outstanding peptide substrate known for calcineurin. The detection of free-phosphate released is based on the classic Malachite green assay and offers the following advantages: Nonradioactive; convenient 1-step detection; excellent sensitivity.