Name: SuR Stranded mRNA-seq Library Prep Kit for Illumina®
Availability: InStock

SuR Stranded mRNA-seq Library Prep Kit for Illumina® | Creative Biolabs

SuR Stranded mRNA-seq Library Prep Kit for Illumina® is suitable for library construction of a strand-specific transcriptome with a starting template of 0.1-1 μg total RNA. Different from the conventional transcriptome library construction, dUTP is labeled in the synthesis of the second strand cDNA; the labeled second strand template is digested with uracil-DNA glycosylase (UDG) before PCR, so that the final sequencing information is from the first strand cDNA, thereby retaining the strand orientation of mRNA. Data analysis after sequencing can determine whether the transcript is from a sense or antisense DNA strand.
Using this kit, the template has a wide adaptability, and moreover, there is a high success rate for plant samples. This product provides optimized disruption and sorting conditions for libraries with inserts larger than 200 bp. Libraries constructed using this product maintain a high degree of consistency with the official Illumina® kit in terms of data output and data quality.