mouse monoclonal IgG2a; HSP 27 Antibody (F-4) is an IgG2a κ mouse monoclonal HSP 27 antibody (also designated HSPB1 antibody, heat shock protein family B (small) member 1 antibody, HSP27 antibody) that detects the HSP27 (HSPB1) protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. HSP 27 Antibody (F-4) is available as both the non-conjugated anti-HSP 27 antibody form, as well as multiple conjugated forms of anti-HSP 27 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress induced by insult. Heat, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. In vitro, HSP 27 becomes highly phosphorylated following exposure to stress. The discovery that HSP 27 is regulated by hormones such as estrogen has led to studies establishing a relationship between HSP 27 and breast cancer.