mouse monoclonal IgG1; Cdc25C Antibody (H-6) is an IgG1 κ mouse monoclonal Cdc25C antibody that detects Cdc25C of human origin by WB, IP, IF, IHC(P) and ELISA. Cdc25C Antibody (H-6) is available as the non-conjugated anti-Cdc25C antibody form, as well as multiple conjugated forms of anti-Cdc25C antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2 in complexes with cyclin E and cyclin A appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase in complexes with cyclin A or cyclin B is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.