mouse monoclonal IgG2a; PAR-2 Antibody (SAM11) is an IgG2a κ mouse monoclonal PAR-2 antibody (also designated PAR2 antibody, Proteinase-activated receptor 2 antibody, or F2RL1 antibody) that detects the PAR-2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. PAR-2 Antibody (SAM11) is available as both the non-conjugated anti-PAR-2 antibody form, as well as multiple conjugated forms of anti-PAR-2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Thrombin receptor (also designated protease-activated receptor-1 or PAR-1), PAR-2 and PAR-3 compose a distinct class of G protein-coupled receptors activated by proteolysis. Cleavage of these receptors by proteases occurs within the amino-terminal extracellular domain. Thrombin, a serine protease involved in platelet aggregation and blood coagulation, activates the thrombin receptor, resulting in elevated intracellular calcium levels in platelets. Thrombin also cleaves PAR-3 in vitro, suggesting that PAR-3 may be involved in thrombosis or mitogenesis. Thrombin receptor and PAR-4 appear to account for most thrombin signaling in platelets. Activation of PAR-2 in vitro is induced by trypsin, suggesting that PAR-2 is not an alternative thrombin receptor (2,6). Cytokines including TNF-α and IL-1β increase PAR-2 expression, indicating PAR-2 involvement in the acute inflammatory response.