mouse monoclonal IgG2b; Amphiphysin II Antibody (99D) is an IgG2b κ mouse monoclonal Amphiphysin II antibody that detects Amphiphysin II splice variants 1-10 of mouse, rat and human origin by WB, IP, IF and IHC(P). Amphiphysin II Antibody (99D) is available as the non-conjugated anti-Amphiphysin II antibody form. Amphiphysin is a brain-enriched protein that exhibits N-terminal lipid interaction and functions as a dimer. Amphiphysin contains a membrane bending BAR domain, a middle Clathrin and adaptor binding domain, and a C-terminal SH3 domain. In the brain, Amphiphysin I and II form heterodimers that bind to the Clathrin associated GTPase Dynamin via their SH3 domains. This association is essential for synaptic vesicle recycling in neurons, as it precedes the binding of Dynamin to the Clathrin-coated pits and the subsequent vesicle budding. In other tissues, Amphiphysin may play a key role in other membrane bending and curvature stabilization events. The mammalian Amphiphysins, Amphiphysin I and Amphiphysin II, have similar overall structure. a ubiquitous splice form of Amphiphysin II that does not contain Clathrin or adaptor interactions is highly expressed in muscle tissue and is involved in the formation and stabilization of the T tubule network.