mouse monoclonal IgG1; HSF1 Antibody (E-4) is an IgG1 κ mouse monoclonal HSF1 antibody (also designated HSF-1 antibody, or heat shock transcription factor 1 antibody) that detects the HSF1 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. HSF1 Antibody (E-4) is available as both the non-conjugated anti-HSF1 antibody form, as well as multiple conjugated forms of anti-HSF1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors 1 and 2 (HSF1 and HSF2), also designated HSTF1 and HSTF2, are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.