mouse monoclonal IgG2b; C3G Antibody (G-4) is an IgG2b κ mouse monoclonal C3G antibody (also designated RPGF1 antibody, Rap Guanine Nucleotide Exchange Factor 1 antibody, Guanine Nucleotide-Releasing Factor 2 (Specific For Crk Proto-Oncogene) antibody, Rap Guanine Nucleotide Exchange Factor (GEF) 1 antibody, CRK SH3-Binding GNRP antibody, GRF2 antibody, C3G antibody, or Guanine Nucleotide-Releasing Factor 2 antibody) that detects the C3G protein of mouse, rat and human origin by WB, IP, IF and ELISA. C3G Antibody (G-4) is available as both the non-conjugated anti-C3G antibody form, as well as multiple conjugated forms of anti-C3G antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Ras p21 is the prototype of a superfamily of GTPases that is involved in the regulation of a wide variety of cellular processes. Ras signals in its GTP-bound form but is "turned off" when bound to GDP. When unregulated or constitutively turned on by mutations, Ras signaling contributes to malignant transformation. The switch between active and inactive Ras is controlled by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). C3G was isolated in a screen for proteins that could bind the SH3 domain of the Crk proto-oncogene product. The carboxy-terminus of the C3G protein displays significant sequence similarity to Ras-GRF/Cdc25Mm and mSos and can substitute for Cdc25 function in S. cerevisiae. These observations strongly suggest that C3G is a GEF for Ras and is involved in the regulation of Ras signaling through Crk. The C3G gene maps to human chromosome 9q34.13 in proximity to the gene that encodes c-Abl, a proto-oncogene that regulates Crk.