Single cell isolation is a necessary step for a variety of applications from developing productive, stable cell lines for biologics manufacturing to developing CRISPR edited iPSCs for disease modeling and pharmaceutical development. Traditionally, manual approaches (i.e. limited dilution) or FACS have been used to isolate single cells to generate clonal cell lines. However, these approaches have high labor demand and low yield and can increase development timelines. The Pala^TM Cell Sorting and Single Cell Dispenser from Bio-Techne automates single cell isolation with dual laser fluorescence and gentle cell dispensing that can improve hit quantity and quality, saving time and yielding better outcomes.

In this webinar, you will learn

• The importance of generating high quality clonal cell lines for manufacturing and research
• An introduction to the capabilities of the Pala Single Cell Dispenser
• Approaches for generating an effective workflow for developing clonal cell lines in your lab

Analyzing protein expression in biological samples has traditionally been challenging and often poorly reproducible. While methods like western blot have been around for decades, Simple Western™ Technology is the modern solution for protein analysis that is easier and faster than other immunoassay methods.

Simple Western is a cutting-edge technology that automates protein analysis, providing high throughput results with significantly reduced sample requirements. Simple Western is a capillary electrophoresis-based system in which proteins are separated by size in a capillary and analyzed using conventional western blot antibodies but without the many limitations of traditional western blots. 

In this webinar, we provide a comprehensive overview of Simple Western Technology and introduce the newest member of the Simple Western family – the Leo™ System. We discuss the applications and benefits for research and development and how Simple Western Technology can provide faster results while significantly reducing errors and variability, making it an ideal tool for multi-sample analysis with analytical-grade quantification. 

Finally, we show how Simple Western Technology can be customized for different research needs, focusing on induced-proximity drug modalities like targeted protein degradation and protein expression potency assays for gene therapy. By the end of this webinar, you will better understand Simple Western Technology and how to use it to revolutionize protein analysis in research and development.

Learning objectives:

• Learn how Simple Western Technology works and enables fast and sensitive protein quantification and detection.
• Learn how the Simple Western Leo System enhances quantitative protein analysis to empower you to design rigorous bioanalytical methods.
• Discuss how Simple Western Technology can be used in induced-proximity drug modalities like targeted protein degradation and protein expression potency assays for gene therapy.