As evidenced by the changes in the new Guide for the Care and Use of Laboratory animals and the latest AVMA Panel on Euthanasia, it is clear that there is growing concern about the care and welfare of aquatic animals used in biomedical research facilities. Although fishes and invertebrates are not covered by the USDA, there are other regulatory bodies, both internal and external, that are adopting this focus. While the vast majority of aquatics used in biomedical research consist of Zebrafish and Xenopus, there are still many other species being used, particularly in coastal research facilities. The use of these animals can include traditional biomedical research, biology research, and aquatic production and research. It can become challenging to accommodate these non-traditional species within established guidelines and regulations, which are mostly geared toward the traditional species listed above. At the University of Miami, we house many different species of marine organisms, each presenting their own unique husbandry and regulatory concerns. This presentation will cover basics such as different housing systems and water supplies, strategic planning of location and species choices, water quality testing protocol selection, IACUC/OB (Institutional Animal Care & Use Committee/Oversight Body) concerns, management issues and topics, exportation of aquatic animals, and resources available for additional testing and guidance. The goal at the end of this presentation is that each facility will be able to devise their own plans to provide high quality animal care while remaining compliant with regulatory bodies. This interactive webinar is designed for laboratory animal professionals at all levels of management and oversight with little, moderate, or advanced experience with aquatics. Learning Objectives: - Similarities and differences aquaculture of non-traditional aquatic species - Internal regulatory handling of non-traditional aquatic species