The routine analysis of steroid hormones is critical in understanding the function of metabolic pathways that impact sexual characteristics, inflammation and blood pressure. Liquid Chromatography-Mass Spectrometry (LC-MS) is fast becoming a sought-after technique in steroid analysis due to the advantages it provides over traditional ligand-binding techniques. These benefits include improvements in analytical sensitivity and selectivity, and the capability of multi-analyte quantitative detection in a single run. However, many LC-MS methods lack harmonization or standardization. The Waters™ MassTrak™ Endocrine Steroid Calibrator and Quality Control Sets (IVD) contain metrologically traceable calibrators, aiding laboratories in their compliance to ISO 15189, and provide confidence in the accuracy of results when using validated LC-MS methods.
In this webinar, the characteristics of the MassTrak Endocrine Steroid Calibrator and Quality Control Sets will be discussed and the performance of an in-house LC-MS/MS method for the analysis of steroid hormones will be demonstrated.
Learning Objectives:
1. Break down metrological traceability that aid laboratories in their compliance to ISO 15189.
2. Describe improvements in laboratory efficiency through the provision of lyophilized materials.
3. Discuss confidence in the accuracy steroid hormones and provide a path to laboratory method harmonization, with excellent lot-to-lot reproducibility.