Introducing LocIn: RoukenBio's new targeted integration system for the efficient creation of isogenic mammalian cell lines

LocIn is an advanced targeted integration system designed for the efficient generation of cell lines by retargeting pre-defined genomic loci, known as "landing pads." These landing pads incorporate a fluorescent protein marker, enabling the easy identification of integrations at genomic sites that support high transgene expression. The system leverages Recombinase Mediated Cassette Exchange (RMCE) to precisely replace the landing pad cassette with a gene of interest. This approach ensures the complete removal of the original cassette during retargeting while exclusively integrating the desired gene. By eliminating positional and context-dependent effects, LocIn enables accurate studies of phenotypic outcomes, delivering reliable genotype-to-phenotype correlations.

Learning Objectives

• Learn what Recombinase Mediated Cassette Exchange is and how it can be used to retarget pre-defined genomic locations (landing pads) for the rapid generation of isogenic cell lines.
• Learn how constitutive EGFP expression from the platform LocIn cell lines means it is possible to assess expression (high/medium/low) potential before retargeting is performed.
• Discover how LocIn’s unique counter selection system ensures that all landing pads are retargeted. Learn how LocIn allows the generation of clonal cell populations without the need for cloning out.

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