Liquid chromatography high resolution time-of-flight mass spectrometry (LC-TOF-MS) is becoming increasingly popular in the clinical research laboratory. It can offer higher throughput and superior specificity to the more traditional tandem mass spectrometry (LC-MS/MS) methods. In addition, LC-TOF-MS has the ability to collect full scan data, which allows for down-stream data mining. This talk will discuss time-of-fight mass spectrometry fundamentals; including theory, method development strategies, pros and cons of LC-TOF-MS as compared to LC-MS/MS, and a few lessons our laboratory has learned along the way. 

Learning Objectives:

• Describe how time-of-flight mass spectrometry works
• Compare and contrast TOF-MS with LC-MS/MS
• Assess what you will need in order to integrate TOF-MS into your laboratory

For Research Use Only. Not for use in diagnostic procedures.