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JUN 24, 2025 9:55 AM PDT

Optimising and validating multiplex qPCR assays

SPONSORED BY: LGC Biosearch Technologies

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Tips for optimising and validating your multiplex qPCR assays

Multiplex qPCR is a popular area of interest for many assay developers. Thanks to distinct reporter dyes, multiple reactions can be combined into a single well, with each target detected independently. This means a single reaction can yield additional data, while reducing the amount of sample required and reagent costs.

However, multiplex assays are inherently more complicated than singleplex, making it crucial to carefully identify the optimal conditions. There is a risk of interactions between multiple primers and probes, or that some reagents may be exhausted quicker than others, leading to unbalanced results.

This ebook is packed with tips covering:

Design considerations unique to multiplex assays
Ensuring all singleplex assays are performing reliably
Common challenges and solutions for optimising in multiplex
Validation and quality control

Get the ebook and enhance your multiplex assays

About the Sponsor

LGC Biosearch Technologies

Biosearch Technologies is the comprehensive genomics portfolio from LGC, providing products and services for genomic analysis that support mission critical applications for global customers in agrigenomics and human healthcare.

The portfolio offers integrated tools and technologies to accelerate sample preparation, amplification, cloning and expression, next generation sequencing (NGS), custom oligonucleotide synthesis, nucleic acid chemistry reagents, and oligo therapeutics.

Whether through sbeadex™ DNA purification, oligos and reagents (KASP™, BHQplus™ Probes, BHQ™ Probe Master Mix), or proprietary sample prep and PCR instrumentation (oKtopure™, SNPline™, Nexar™ and IntelliQube™), we enable our customers from discovery to production-scale testing.

Biosearch Technologies is a leader in the agricultural genomics market and is increasingly adopted by researchers, with over 2,000 scientific papers citing KASP assays. Key applications include validation and screening of newly discovered SNPs in Marker-Assisted Selection and Marker-Assisted Breeding programmes for both plants and livestock.

Globally, our cGMP manufacturing and ISO 13485-compliant facilities support supply chain continuity, complementing our customers' product development pipelines through our robust research grade and cGMP oligonucleotide manufacturing that achieves consistent scale up. For molecular diagnostics, Biosearch Technologies has proven expertise in NGS, isothermal, qPCR-based applications where our tools and technologies such as BHQ probes and proprietary PCR platforms are frequently used in LDTs, ASRs and in vitro diagnostics. Through our recently acquired Lucigen portfolio, we also offer custom and bulk enzymes and buffers as critical components for molecular diagnostics.

Sales offices are located in China, Germany, Singapore, UK, and US. Sales representatives are available throughout Europe, Latin America, and Asia Pacific, with distributors in other key territories. Visit www.biosearchtech.com