Next-Level Protein Purification: Speed Meets Precision

Purifying proteins efficiently is critical for downstream analysis and assay development. This application note shows how combining automated liquid handling with affinity-based purification can streamline workflows and improve reproducibility.

Inside this application note, you’ll learn:

•  How affinity resin in PhyTip^® columns enables selective capture and elution of target proteins
•  Why automated workflows reduce manual steps and improve consistency in protein purification
•  How histidine-tagged proteins are purified using IMAC resin in a fully automated process
•  Key performance metrics: processing time (~120 min), protein yield, and purity assessment
•  Practical insights from Bradford assay, SDS-PAGE, and mass spectrometry validation

Whether you’re optimizing protein isolation for research or scaling up for high-throughput applications, this guide provides actionable strategies to simplify purification and ensure high-quality results.