Human-Induced Pluripotent Stem Cell Expansion in a Stirred 3D System: an Example of Manufacturing Allogeneic Resources for Cell Therapy

Allogenic cell therapies require the constant supply of billions of cells generated by robust, and economically viable bioprocesses. Human induced pluripotent stem cells (iPSCs) are a unique source for the production of functional human cell types, fueling advanced regenerative therapies. Single-Use stirred tank bioreactors have the potential to become the new gold standard for the commercialization of allogeneic cell therapy production. We here show, how we instrumented stirred tank bioreactor technology to take iPSC cultivation to the next level.

In this talk, we want to provide a deeper understanding of hPSC bioprocessing and how single-use stirred-tank bioreactors can aid in achieving this goal. Innovations in impeller designs improve the cultivation of stem cells as aggregates or on microcarriers. By precisely monitoring and controlling critical process parameters, we will show how bioreactor processyields can be significantly increased.

Learning Objectives:

1. Define basic principles of stem cell bioprocessing in stirred systems.

2. Identify the importance of process monitoring.

3. Process optimization: Discover the different possibilities of parameter control.