Purifying Tagless Recombinant Proteins with a Disruptive Self-Removing Affinity Tag Platform

Self-removing affinity tags have the potential to greatly accelerate the development of new therapeutics, while providing a potentially enabling manufacturing platform for non-mAb therapeutic proteins.  One example involves the pH-controlled self-cleaving of a small intein-derived affinity tag.  In this talk, I will present our most recent work with our tag, including case studies on the purification of a variety of biosimilar candidates and potential biopharmaceutical candidates.

Learning Objectives: 

1. Review how self-removing affinity tags are an effective way to purify non-mAb proteins.

2. Summarize how basic protocols for using self-removing tags will be provided.

3. Explain how properties of the target proteins to be purified can have a significant impact on the cleaving rate.