Single Molecule Light Field Microscopy, a Revolution in 3D Detection

Light field microscopy (LFM) is a revolutionary technique first introduced in 2006 which can essentially capture a 3D volume in a single snapshot, complete with digital refocusing and deconvolution. Typical 3D imaging in microscopy requires multiple captures that are digitally combined post-capture, but with LFM 3D imaging can be done live, at high speed, enabling the capture of more than ever before.

 

The group of Prof. Steve Lee (University of Cambridge) is applying LFM to single molecule localisation microscopy, resulting in fast, high-density volumetric imaging of single molecules. With just an array of 7 microlenses, courtesy of Cairn Research, Prof. Steve Lee can perform 3D super-resolution imaging!

 

In this webinar you'll hear all about LFM as a technique, it's applications, and how guest speakers Prof. Steve Lee and Dr. Sam Daly from The University of Cambridge are revolutionizing 3D single molecule microscopy. This webinar also features Jeremy Graham of Cairn Research as a guest speaker, to describe the design of the 7 microlens array that makes LFM possible. To finish off, we explore how LFM requires high sensitivity, large pixel array, high speed CMOS cameras, such as the Kinetix CMOS from Teledyne Photometrics, and how we have developed custom software for easy LFM image processing using our very own PySMLFM open source Python script.

 

Tune in to hear all about the future of 3D microscopy!

 

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