Spatial Context Matters: Molecular Cartography™ to Study Gene Expression Changes in the Brain

Technological advances such as single-cell RNA sequencing accelerated our understanding of cellular diversity in tissues. However, the ability to elucidate this cellular heterogeneity while retaining spatial information is crucial for understanding the complex biological networks in health and disease. At Resolve Biosciences we developed Molecular CartographyTM, a highly multiplexed, single-molecule detection technology to measure transcriptomic activity with full spatial context at subcellular resolution. The unparalleled sensitivity and specificity of Molecular CartographyTM helps scientists detect individual transcripts and rare signals to interpret fundamental biology and rapidly advance the understanding of comple x biological questions in neuroscience. In this presentation, we demonstrate applications in mouse and human brain tissue in which we detect millions of individual transcripts originating from a hundred genes in each sample. This allows us to unravel the complex spatial organization of different cell types and their changes in gene expression in response to pathology, for example, in the context of Alzheimer's disease.

Learning Objectives:

1. Method for highly multiplexed and sensitive spatial transcriptomics

2. Spatial analysis of cell phenotypes in response to pathology in the context of Alzheimer’s disease