Visualizing Cancer Biology through Cell Culture Inserts

Understanding cell-to-cell interactions in a tumor microenvironment are increasingly challenging due to the complexity of cancer cells. Existing cell culture methods to visualize cancer cell interactions are limited to mono-layer culture with few established co-culture techniques. Cell culture inserts are unique vessels separated by a porous membrane, designed to maintain cells in two different layers, which enables the study of specific cell-cell functions, including ion and molecular transport, migration, and invasion in various cell types. In this presentation, we outlined several co-culture and triple culture methods to effectively visualize cancer hallmarks using the cell culture inserts system.

 

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