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Recent advances in high-throughput single-cell transcriptomics and epigenomics methods allow to measure the expression of thousands of genes and identify the gene transcriptional regulation...
Learning Objectives: 1. Using an optics- and label-free approach to CRISPR research 2. Explain current approaches to selecting the most robust gRNA for CRISPR assays 3. The opportunities tha...
The current gold standard for COVID-19 diagnosis is based on the detection of SARS-CoV-2 nucleic acid using real-time quantitative PCR (RT-qPCR). Recent studies suggest limitations of RT-qPC...
The major function of mitochondria in cellular homeostasis has been the generation of ATP through oxidative phosphorylation. However, we have previously demonstrated that mitochondria can se...
Cancer is complex, but recent findings are yielding a greater understanding of the disease. The tumor suppressor genes BRCA1 and BRCA2 are implicated in breast, ovarian, prostate, and other c...
Next-generation sequencing is transforming scientific research and testing. Thanks to large-scale cancer projects (e.g. The Cancer Genome Atlas - TCGA), the accumulated knowledge is now used...
Learning Objectives: 1. Demonstrate the utility of long-read RNA-sequencing in studying alternative isoforms 2. Define the long-Split-seq approach to profiling the transcriptomes of single-c...
Directed evolution is an effective strategy to optimize the properties of proteins for specific requirements, either for industrial, therapeutics or research applications. As the theoretical...
The host antiviral innate immune response involves activation of multiple signaling pathways that result in the production of type I interferons (IFN-I) and inflammatory cytokines, which tog...
Learning Objectives: 1. Understand the viral kinetics and impact on testing for patients with COVID-19. 2. Understand available testing options. 3. Be able to determine positive and negative...
Recent advances in machine learning have shown that deep neural networks (DNNs) can provide powerful and flexible models of neural sensory processing. In the auditory system, standard linear...
Polypharmacy, the simultaneous use of multiple medications, is growing at an alarming rate with reports documenting a range of 12 to 22 prescriptions being used on average by individuals >...
Cancer disparities among persons of African descent are driven by both biological and nonbiological factors. There is evidence in breast cancer that psychosocial factors (environment, socioe...
Rare disease affects 30 million people in the United States, with nearly 50% of cases affecting children. Because nearly 80% of rare diseases are genetic, advancements in genomic testing are...
Reactive oxygen species (ROS) are well-known for their detrimental effects leading to oxidative stress, cell death, aging, and degenerative disorders. However, there is increasing evidence t...
Ebola virus executes its replication cycle through a tightly orchestrated sequence of events, but the host factors that govern the timing of these steps have remained poorly defined. In this...
Over the past ten years, CRISPR technology has revolutionized genome engineering, as reflected by the recently awarded Nobel Prize. Nevertheless, the process of performing and characterizing...
Targeted sequencing has many applications in cancer biomarker research, carrier screening and inherited disorders, drug development, mitochondrial DNA variant detection, human ID and paterni...
Programmed death-1 (PD-1) inhibits T and B cell function upon ligand binding. PD-1 blockade revolutionized cancer treatment, and while numerous patients respond, some develop autoimmune-like...
To prepare for the 2019 flu season it is important to get immunized. In 2019 a tetravalent (four-virus) vaccine is available in a number of forms including an egg-free formulation that is for...
COVID-19 is a severe disease that has caused >1 million deaths in under one year. As this disease is novel, the molecular and cellular underpinnings of the progressive tissue injury are p...
Analytical Method Validation work is based on a variety of guidelines and requirements hence being time-consuming and stressful. Reducing this work and improving efficiency while ensuring th...
My group is addressing fundamental questions in evolutionary biology, using both genome- and phenotype-first approaches. A few years ago, we discovered that Arabidopsis thaliana is a great m...
Safety and potency testing in the cannabis and hemp industries necessitates analytical systems that include chromatography, mass spectrometry, and real time quantitative polymerase chain rea...
Within the clinical arena, the oncology community has led the way in the early adoption of next generation sequencing. NGS is in widespread use in clinical cancer research and is now beginni...
It has been noted by many in the community that for Precision Medicine to become a transformative reality, the underlying DNA and RNA sequence data have to become more precise. In my talk, I...
Drug overdose surpassed motor vehicle accidents as the leading cause of accidental death 2006 and has remained the leading cause of accidental death at the state and national level. Mos...
Recent advances in high-throughput single-cell transcriptomics and epigenomics methods allow to measure the expression of thousands of genes and identify the gene transcriptional regulation...
